Reporter

Part:BBa_K2760029:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-11)


M-cherry optimized for both L.rhamnosus and E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    • Can be used for L. rhamnosus or E. coli



Source

This sequence is a modified version of BBa_J18932, for codon optimization purposes bases were changed in order to allow the expression in both L. rhamnosus and E. coli. It also contains a 6-His-Tag to ameliorate purification processes, the sequence for this optimized M-cherry can be taken from the sequence page and another user could omit the His-Tag if it is not needed.

References